Increased vascular permeability to evans blue dye in the hippocampus of PDAPPJ20 mice, model of Alzheimer´s Disease (AD). Potential implication of ER Stress mechanisms

PRESA JL; POMILIO C; ALAIMO A; BENTIVEGNA M; VINUESA A; GREGOSA A; RAMHORST R; PEREZ OE; BEAUQUIS J; SARAVIA F

Mar del Plata

Congreso; LXIV Reunión Científica Anual Sociedad Argentina de Investigación Clínica.; 2019

Sociedad Argentina de Investigacion Clinica (SAIC)

The blood-brain barrier (BBB) limits flux from and into the brain compartment that is essential for normal neuronal functioning and information processing. Post-mortem tissue analysis indicates BBB damage in AD. However, timing and mechanisms underlying BBB breakdown remain elusive. Endoplasmic reticulum (ER) stress is caused by disruption of homeostatic mechanisms that cause unfolded proteins to accumulate. The ER adapts to stress by activating the unfolded protein response (UPR). Chronic ER stress is increasingly being associated to neurodegeneration. We found augmented vascular permeability in the hippocampus of 12-month-old PDAPPJ20 transgenic mice, compared to the group, assessed by Evans Blue e.v. injections and analysis of coronal brain sections (D.O.=5666 +/- 387 vs 12373 +/- 3082 n=5-6 p < 0.04) and a higher number of microhemorrhages per um2 in the hilium/four fold more in the transgenic mice vs nos-transgenic micr p