Nanoecapsulation of nutraceutical compounds and bionanoencapsulation of proteins and nuclei acids

PEREZ OE; CORFIELD R; DOMINGUEZ RUBIO AP; ALAIMO A; MARTINEZ JH

Praga

Congreso; 3rd World Congress on Proceedings of Nutrition, Dietetics and Nutraceuticals; 2019

EuroSciCon

Growing attempts are being made to rationally utilize systems for humanhealth improvement and disease prevention. In this context, thiscontribution is part of our interest in use nano-encapsulation strategies toconstruct nano-vehicles; which in turn will exert the controlled and targeteddelivery of nutraceuticals. A nutraceutical can be defined as the actualsubstance that confers a health benefit. Our research group has carried outthe nano-ecapsulation of bioactive compounds as resveratrol (Res), folicacid (FA), betanin (Bt) into different biocompatible matrices. We also studiedthe microvesicle production (MV) by Lactobacillus casei BL23 involved in itsprobiotic activity.We characterizes the interactions between β-lactoglobulin(β-lg) and isolated 7S and 11S globulins obtained from defeated soy flourand folic acid (FA) at different load ratio and their functional implications, in terms of colloidal behaviour and digestibility. The biological activity of nanocomplexes loaded with FA was explored in terms of their capacity to enhancethe biomass formation of L. casei BL23. The results concerning to nanocomplexesinclusion in culture media showed higher bacterial growth. Bt loadedin a nanovehicle of 11S quinoa seed protein was designed. At this respect, wefound that protein solubility was increased in the presence of Bt. 11S-Bt nanocomplexes showed a synergistic effect in terms of both, antiradical or reducingpower capacity in comparison to Bt as evaluated by to methods, 2,2 -azino-bis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), and by ferric reducing antioxidantpower (FRAP). On the other hand, Res was included into nanoparticles ofchitosan, a mucoadhesive and positive charged polysaccharide. Bioactivityof all these nanoencapsulated materials were evaluated by in-vitro assaysof different human culture cells lines. These nanoencapsulated materialscould have the potentiality to exert the bioactive protection and the controlleddelivery in food, pharmaceutical and nutraceutical products. MV could be usedas nanomachine controlling the host genes expression.