Cytotoxicity assesment of hydroxiapatite nanoparticles in normal and tumos cell lines

GOROJOD RM; ALAIMO A; PORTE ALCON S; DITTER ML; GONZALEZ MC; KOTLER

Mar del Plata

Congreso; LXI ANNUAL MEETING ARGENTINE SOCIETY FOR CLINICAL INVESTIGATION (SAIC); 2016

ARGENTINE SOCIETY FOR CLINICAL INVESTIGATION (SAIC)

Hydroxyapatite (Ca10(PO4)6(OH)2; HAP) is an essential component of the human bone inorganic phase. Nanoscaled HAP (nHAP) presents emergent properties in comparison to its bulk counterpart, including an inhibitory effect on the proliferation of some types of tumor cells. However, little is known regarding to the potential adverse effects on human health. In this study, we aimed to determine the cytotoxicity of nHAP in both normal and tumor cell lines. For this purpose, we evaluated cell viability (MTT assay) after exposure to nHAP (50-200μg/ml, 24-72h) in both normal (fibroblasts NIH/3T3, microglia BV2 and retinal pigment epithelial cells ARPE-19) and tumor cell lines (astroglia C6, liver HepG2, lung A549 and epithelial HEp-2). Some cellular lines were selected for additional evaluation: lysosomal and nuclear integrity (LysotrackerRed DND-99 and Hoechst 33258 staining, respectively) and cell proliferation by clonogenicassay and Ki67 immunolabeling. Data obtained demonstrated a cytotoxic effect in C6 and HEp-2 cells after 24h exposure (C6: 100μg/ml: 14±5% p