LOCAL EFFECTS OF LOW DOSES OF PTH 1-34 ON EXPERIMENTAL PERIODONTITIS

ZENI,SUSANA; PRELIASCO,MARIANA ; BIDEVICH,NICOLAS; MARIO RICARDO DAVISON; BONAMO, MARIANA

CABA

Congreso; Reunión de las Sociedades de Biociencias 2020.LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica. SAIC; 2020

SAI- SAIC- SAFIS

67. (286) LOCAL EFFECTS OF LOW DOSES OF PTH 1-34 ON EXPERIMENTAL PERIODONTITIS
Susana Noemi Zeni (Osteology and Bone Metabolism Lab. INIGEM. School of Biochemistry. Clinical Hospital. (UBA/ CONICET)), Mario Ricardo Davison (Dentistry School. Rio Negro National University.), Nicolas L Bidevich (Dentistry School. Rio Negro National University.), Mariana Preliasco (Dentistry School. Rio Negro National University.), Marina Bonanno (Osteology and Bone Metabolism Lab. INIGEM. School of Biochemistry. Clinical Hospital. (UBA/CONICET)) INIGEM (UBA/CONICET) Periodontitis is a highly prevalent, chronic disease that induces a progressive bone resorption. Intermittent PTH administration has anabolic and anti-in ammatory effects, two properties necessary to achieve bone recovery. Periodontal disease can be experimentally induced in rats in few days, via cotton ligatures placement in the gingival sulcus around the molar teeth, that increases bio lm accu- mulation and disruption of the gingival epithelium, enhancing osteo- clastogenesis and bone loss. We investigated whether intermittent administration of a low dose of PTH 1-34 in rats would block the alveolar bone loss observed when the ligature model of periodon- titis was used. Periodontitis was induced in 16 female Wistar rats (221±15g) under light anesthesia. Ligature was replaced every 4 days. Rats were randomly divided in two groups and subcutane- ously injected every 48 hs for 18 ds. with: G1 saline solution and G2 1.2ug PTH1-34. Eight rats were left unligated as healthy con- trol. After killed hemimandibles were extracted and xed in forma- lin buffer for histologic analysis of tibia subchondral bone volume (BV/TV%), alveolar bone BV/TV% and periodontal space height (PSH). Results: tibia BV/TV%: C 38.77±2.59; G1 38.29±3.9 and G2 37.75±1.45; alveolar bone BV/TV% C 50.3±3.6c; G1 35.6±4.3a and G2 42.0±1.4b; PSH (mm): C: 0.196±0.057a; G1 0.809±0.115b and G2: 0.706±0.065c. Different letters show a p