Nonalcoholic fatty liver disease: resistin differentially regulates T cell activation and reactive oxygen species levels

A. C. CHERÑAVSKY; COLOMBATO LUIS; ALEGRE NADIA S.; PONCINO DANIEL; BAZ PLACIDA; GARCIA DANIEL; BENAVIDEZ JAVIER; CECILIA GARCIA

Buenos Aires

Congreso; Reunión conjunta de sociedades de Biociencias; 2017

Sociedades de Biociencias

Resistin (RES) is a cytokine which plasma concentration has been found elevated in Nonalcoholic Fatty Liver Disease (NAFLD) patients. Although human RES is produced by immunological cells, its effect on them are poorly understood. We have previously demonstrated that RES decreases CD69 expression in activated T cells from controls (Co) but not from NAFLD patients. We aimed to evaluate RES-mediated modulation of CD25 in T cells from NAFLD patients and Co and RES ability to modulate reactive oxygen species (ROS) production in peripheral blood mononuclear cells (PBMC). PBMC were obtained from NAFLD (n= 9) patients and Co (n= 14). Isolated T cells were activated with coated anti-CD3 (3 ug/ml)+/-RES (10 ng/ml) for 72 h, stained with anti-CD4, -CD8 and -CD25 mAbs and evaluated by flow cytometry (FC). To evaluate ROS levels, PBMC were incubated with or without RES (20 ng/ml) for 24 h, stained with 2´7´-dichlorofluorescin diacetate (DCFH-DA) and anti-CD3, -CD4, -CD8, -CD14 mAbs and studied by FC. As a preliminary approach, monocytes oxidative burst was stimulated with PMA (100 ng/ml)+/-RES, and ROS production was evaluated by DCFH-DA. Mann-Whitney and Wilcoxon paired tests were used. RES decreased CD25 expression in activated T cells from Co but not from NAFLD patients. As a result, CD25 expression is higher in CD4+ (p