TLR4 and TLR9 expression might molulate the adaptive immune response in experimental nonalcoholic steatohepatitis

ALEGRE NADIA S.; INZAUGARAT ME; CECILIA GARCPIA; A BILLORDO; DE MATTEO,E; WALD M; A. C. CHERÑAVSKY

CABA

Congreso; First LASID-SAI-FAIC MEETING; 2015

SAI-LASID-FAIC

Background: Toll like receptors (TLRs) expression in T cells allows the modulation of the adaptive immune response. We have demonstrated that Curcumin (Cur) oral treatment modulates intrahepatic immunological activity in a high fat diet (HFD)-induced model of nonalcoholic steatohepatitis (NASH). We aimed to evaluate TLR4 and TLR9 expression in T cells from liver and spleen, the association between TLRs and tissue injury and the in vivo Cur effects in HFD-fed mice.Methods: C57BL/6 mice were fed normal chow (NC group) or high fat diet (HFD group) (10 mice/group). After 4 weeks of feeding, Cur (2g/kg diet) was co-administered to half mice of each group (NC+Cur, HFD+Cur groups). Mice were sacrificed and then spleen and liver were weighed. Cell suspensions were obtained and stained with specific anti-CD4PE/APC, -CD8APC, -TLR4PE and -TLR9FITC antibodies for analysis by flow cytometry. Liver nonalcoholic fatty liver disease activity score (NAS) was also assessed. %TLR4+ cells , and TLR9 mean fluorescence intensity (MFI) were analyzed in CD4 and CD8 positive cells by two-way ANOVA with Bonferroni´s post-test. Spearman´s test was used for correlations. Results: HFD increased spleen (TLR4+CD8+: p