EFFECT OF FEEDING A LOW LACTOSE YOGURT-BASE DIET HAVING GALACTOOLIGOSACCHARIDES (GOS) ON BONE HEALTH: PRECLINICAL MODEL OF NORMAL GROWTH

SEIJO, MARIANA; VÉNICA, CLAUDIA INÉS; BERGAMINI, CARINA; ZENI, SUSANA NOEMÍ; ZENI CORONEL, MAGALI; BOZZINI, CLARISA ; PEROTTI, MARÍA CRISTINA; BONANNO MARINA SOLEDAD; DE PORTELA, MARÍA LUZ PITA MARTÍN; WOLF, IRMA

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2021 vol. 81

0025-7680

GOS are natural prebiotics of human milk that could have positive actions in calcium (Ca) absorption and bone health. They can be incorporated in fermented dairy products by enzymatic action on milk lactose, resulting in a low lactose-containing food.We evaluated and compared the effect of feeding a low lactose-yogurt containing GOS (EY) in bone health during normal growth of weaning rats. Rats (n=10/group) fed 3 diets: control AIN?93-G (C);GOS-free yogurt (Y) or EY during 30 days.We evaluated food consumption; body weight (BW); Ca absorption (% AbsCa) by balance methods; in cecum Lactobacilli growth (LB) by microbiological culture, pH by a pHmeter and short chain fattyacids (SCFA) by HPLC-IR; total skeleton (Et), lumbar spine (Ls) and proximal tibia (Pt) bone mineral density (BMD) and Et bone mineral content (BMC) by densitometry; bone volume (%BV) and intestinal crypt depth (ICD) (µm) by histology; maximal load, fracture strength and elastic modulus by biomechanical test. ANOVA and Bonferroni post hoc test were used to evaluate statistical significances.Food consumption and BW were similar thought out the study. EY showed higher %AbsCa (p