RADIOPROTECTIVE EFFECT OF VEGETABLE OILS IN AN EXPERIMENTAL MODEL OF ACUTE RADIATION SYNDROME

LOPEZ G; COSTANTINI MH; CASTRO GD; CASTRO JA; MONTALTO DE MECCA M; FORMOSA LEMOINE F; DIAZ GOMEZ MI; MACIEL ME; LOPEZ G; COSTANTINI MH; CASTRO GD; MONTALTO DE MECCA M; DIAZ GOMEZ MI; CASTRO JA; FORMOSA LEMOINE F; MACIEL ME

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC,SAIB,SAI,SAA,SAB(Biofísica), SAB(Biología), SAFE,SAFIS,SAH,SAP

In the present study, the radioprotective effect of several vegetable oils containing different amounts of polyunsaturated fatty acids, tocopherols and tocotrienols were tested using an experimental model in Sprague-Dawley rats (both sexes) exposed to X radiation (2 Gy, whole body). Groups of 8 animals were exposed at a dose of 2 Gy. Blood samles were obtained after 48 hours by caudal puncture, followed by sampling at 7, 14, 21, 28 and 60 days. The erythrocyte, leukocyte and leukocyte formula were counted. Survival curves up to 60 days were also performed. Genotoxic effects in leukocytes were assessed by the Comet assay one hour post-irradiation. Oils (wheat germ, rice seed, palm, grape seed, chia, avocado and olive) were administered subcutaneously two hours before irradiation at a dose of 1 g/kg. For comparison, the effect of alpha-tocopherol was tested at a dose of 570 mg/kg (i.p.) administered 4 hours prior to irradiation, and also for its acetate (given at a dose of 750 mg/kg (i.p.) 24 hours before irradiation). In irradiated animals erythrocytes were significantly depleted (females, p