RADIOPROTECTIVE EFFECT OF SODIUM ALIZARINSULFONATE IN AN EXPERIMENTAL MODEL OF ACUTE RADIATION SYNDROME

M.H. COSTANTINI; M. MONTALTO DE MECCA; G.D. CASTRO; F. FORMOSA LEMOINE; M.I. DÍAZ GÓMEZ; M.E. MACIEL; G.D. LÓPEZ; J.A. CASTRO

Goiania

Congreso; XX Congresso Brasileiro de Toxicologia; 2017

Sociedade Brasileira de Toxicologia

Increased intracellular calcium is a factor known to be involved in the process leading to cell death. Changes in calcium homeostasis are relevant to the late stages of cell injury. Sodium alizarinsulfonate (ASR) has a potent action as calcium chelator, thus interfering with the entrance and action of this element in the cell. It is our interest to develop less toxic radioprotectors, either by themselves or as adjuvants to drugs already approved for their use in humans but having toxic side effects. In this work, using an experimental model of Sprague-Dawley rats (both sexes) exposed to X radiation (2 Gy, whole body), the radioprotective effect of ASR was evaluated. Groups of 8 rats were exposed at a dose of 2 Gy. At 48 hours post exposure blood samples were obtained by tail puncturing, followed by sampling at 7, 14, 21, 28 and 60 days. The erythrocyte, leukocyte and leukocyte formula were counted. Survival curves up to 60 days were also performed. Genotoxic effects in leukocytes were assessed by the Comet assay (one hour post irradiation). The effects of ASR were tested following its administration as a single dose of 100 mg/kg (i.p.), one hour before irradiation. In the irradiated animals erythrocytes were depleted (females, p