IN VIVO ASSESSMENT OF CLOSANTEL OVICIDAL ACTIVITY IN FASCIOLA HEPATICA EGGS

SOLANA M.V.; NEIRA, N.; SCARCELLA S.; MERA Y SIERRA, R.; SOLANA|| H.

EXPERIMENTAL PARASITOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2016 vol. 160 p. 49 - 53

0014-4894

The trematode Fasciola hepatica is a cosmopolitan parasite which causes considerable loss in sheep and cattle production systems all over the world. Chemotherapy is the main tool available to control of the liver flukes. The anthelmintic Closantel, is effective against a wide range of hepatic and intestinal trematodes in a variety of animals. It highly effective against F. hepatica with both an in vivo e in vitro efficacy against mature and immature stages, does not exhibit ovicidal activity in vitro. The ovicidal effects in vivo at present are undetermined. In the present work, modified egg hatch assay (EHA) was used to evaluate the in vivo ovicidal activity of Closantel on fluke eggs. For this purpose the exposition at the anthelmintic Closantel was at the host. At 12, 24 and 36 h post-treatment he eggs collected of the gall bladder was observed and immediately incubated without added drug at the assay. Regarding the morphometric analysis of the eggs, between experimental and control groups there were statistically  significant differences regarding width and shape yet no significant differences were found in length and size. Eggs exposed to the effect of closantel tend to be more elliptical. Significant differences were found in the hatching of eggs between 36 hs post treatment (32, 5%) vs. aproximately 85% in control,12h and 24 h post treatment. This work confirms Closantel affect in vivo the normal development of the eggs. As one of the first effects, this drug affects the performance of the trematode´s reproductive physiology. These results have an impact on the interpretation of the Faecal Egg Counts in efficacy studies and may be useful to further understand the mechanisms underlying the drug activity in target helminthes parasites