Studies pharmacokinetic and phototherapeutic of CP and CF3 porphyrins in vivo: application of photodynamic therapy.

M. G. ALVAREZ, B. RUMIE VITTAR, M. E. MILANESIO, E. N. DURANTINI, M. BERTUZZI, V. RIVAROLA

Córdoba

Congreso; Asociación de Bilogía de Córdoba; 2004

Photodynamic therapy (PDT) is emerging as an alternative modality for cancer therapy. It induces neoplastic cell death for photoachievable sensitizers. It offers improved selectivity for targeting tumor compared with conventional chemotherapy and radiotherapy. We have analyzed the photosensitizing properties of two porphyrin: 5-(4-N-(2´,6´-dinitro-4’-trifluorophenyl)phenyl)-10,15,20–tris (4-trimethoxyphenyl) porphyrin (CF3) and 5-(4-trimethylammoniumphenyl)-10-15,20-tris (2, 4, 6-tri-methoxy-phenyl)porphyrin (CP) on cell cultures. In darkness, CF3 and CP did not alter the survival of cells cultures, but produced mortality cellwhen irradiated. The aims of this work were evaluate the photodynamic effects of CF3 and CP on Balb-c rats. The photosensitizers are accumulated in liver and duodenum. They are eliminated from the body, via bile-gut. They do not cross the blood-brain barrier and do not produce toxicity in the skin. In phototherapeutic studies,it was observed almost complete tumor ablation (90%) for tumor injected with CP after 7-15 days post-irradiarion, while CF3 was not observed significant regression. The results show that CP could be adequate sensitizer for PDT.