PRL-3 expression in human pituitary adenomas and prolactinoma experimental model: a possible role in the tumoral development

GABRIELA DEISI MOYANO CRESPO, CAROLINA GUIDO, PABLO A. PÉREZ, SILVINA GUTIÉRREZ, FLORENCIA PICECH, JUAN P. PETITI, ALICIA I. TORRES, JORGE H. MUKDSI

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica

PRL-3 (phosphatase of regenerating liver 3) is a subclass of proteintyrosine phosphatases involved in proliferation, migration, invasionand growth tumor, and have been proposed as potential biomarkersof tumor progression. Our aim was to investigate whether PRL-3 isexpressed in pituitary human tumor lesions evaluating its presenceand correlating with some clinical-pathological parameters. Besideswith the objective to analyze a possible PRL-3 participation in pituitarytumorigenesis, this phosphatase and p21 were evaluated in alactotroph tumoral experimental model. We analyzed PRL-3 in 46human pituitary adenomas and Fisher male rats treated with estradiolbenzoate (EB) during 10, 20 and 30 days (n: 9) were used. Normalhuman (n: 2) and rats (n: 3) pituitaries were use as control group(CG). Immunohistochemical technique was applied at photonic andelectronic level and a semiquantitave study by western blot wasused. Statistic analyzes: x2 for the variables: size, function and ki67 in human adenomas. Anova-Fisher and correlate analyze in theexperimental model. From the total human adenomas, PRL-3 wasexpressed in 22 out of 46 with immunolocalization predominantly incytoplasmic level. Interestingly at ultraestructural level some tumorsexhibited phosphatase immunolocalization at plasma membrane.There was a positive relation with tumor size (macroadenomas, x2p