Signs of alterations in the mitochondrial dynamic and oxidative stress in the senescence process during the development of estrogen-induced pituitary tumors

GRONDONA EZEQUIEL; CARREÑO LUCIA; SILVINA GUTIERREZ; DE PAUL ANA L; MONGI BRAGATO BETHANIA; PETITI JUAN PABLO; LATINI ALEXANDRA; SABATINO MARÍA EUGENIA; SOSA LILIANA DEL VALLE; TORRES ALICIA I

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2016

Sociedad Argentina de Investigación Clínica (SAIC).

Evidence of cellular senescence process during in vivo estrogen-induced pituitary tumor development was recently described in our laboratory. Since mitochondrial metabolism and dynamic are targets of estrogen action and senescence is considered a stress response triggered by different factors including oxidative stress; we aim to evaluate the effects of estrogen in vivo on mitochondrial function and dynamics in experimentally induced proliferative lesions.To induce pituitary tumoral development, Wistar male rats were exposed to estradiol benzoate (30mg) implanted subcutaneously in silastic capsules for 10, 20, 40 and 60 days (E10-60). Control group: animals treated with empty capsules. The morphological and morphometric mitochondrial analysis was evaluated by transmission electron microscopy; ROS production and mitochondrial membrane potential was determined by flow cytometry. Mfn1, Mfn2, OPA-1, Drp1; 8OHdG and Nrf2 protein expression were assessed by immunohistochemistry and western blot. Statistical analysis: ANOVA-Fischer test (p