Dietary n-3 fatty acid deficiency induces depression-related behavior mediated by vasopressin

BIANCONI S; SANTILLAN ME; WILLIAMS M; MAESTRI G; SCHIÖTH HB; CARLINI VP; PORETTI MB; SOLIS MR; STUTZ G

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAIC, SAI, SAFE

Adverse fetal and postnatal conditions, such as nutritional imbalance, can predispose to psychiatric disorder development in the adulthood. Omega-3 (n-3) polyunsaturated fatty acid (PUFA) offer can modify the central nervous system development and function. Thus, we propose to study the effect of long term exposition to different dietary levels of n-3 PUFA, from gestation to adulthood, among depression-related parameters. Three groups of albino Swiss female mice (during gestation-lactation) and then their male offspring (during weaning-adulthood; n~10 per group) were fed with C (control, adequate in n-3 diet; 7% soya oil;n-3:0.57%; n-6/n-3: 5.7), D (deficient in n-3 diet; 7% sunfloweroil; n-3: 0%;n-6/n-3: 0) or E (excessive in n-3 diet; 7% blend oil: cod liver 60% + soya 40%; n-3: 1.25%; EPA 0.43% y DHA 0.32%n-6/n-3: 1.29). At postnatal day 63,open field test was performed in order to study locomotor activity. Then, animals were sacrificed to determine plasma corticosterone levels by ELISA and gene expression of vasopressin (AVP) and corticotropin releasing hormone (CRH) -in hypothalamus- and their receptors AVPr1b, CRHR1 and CRHR2 -in the pituitary gland- by RT-qPCR. The D group showed higher locomotor activity (crossing number: D 415 ± 37 vs C 195 ± 25; p