Renal Oxidation of ESS diabetic rats is minimized by EPA

GARCÍA N; OSIEKI N; DIAZ-GEREVINI GT; RUIZ PECCHIO A; EYNARD AR; REPOSSI G

Amsterdam

Simposio; 17th INTERNATIONAL SYMPOSIUM ON ATHEROSCLEROSIS; 2015

International Atherosclerosis Society

Salt sensitivity (SS) is associated with increased cardiovascular risk in diabetic patients due toincreased renal oxydation and decreased urinary sodium excretion. Western diet has limitedcontent of w3 polyunsaturated fatty acids, with antioxidant capacity, such as ecoisapentaneoicoacid (EPA). Therefore we hypothesized that nutritional supplementation with EPA, prevents SS inDM rats by decreasing renal oxidative stress. Methods: Wistar rats were used as healthy controls.Type II diabetic rat group (eSS), 3 months old, were divided in 3 groups, diabetic control (eSS),eSS treated with arachidonic acid (pro-oxidant)(2.5mg/ip, monthly) (eSS+AA) and eSS treatedwith EPA (2.5mg/ip month) (eSS+EPA). Animals were treated during 1 year, then placed inmetabolic cages and subsequently underwent 2 subsequent experimental periods of 7 days eachwith normal sodium diet (0.4% NaCl)(NNaD) and high salt diet (4% NaCl)(HNaD). At the end ofeach period, weight, systolic blood pressure (SBP), HbA1c, triglycerides (Trig), cholesterol (Chol),creatinine (Cre), kidney γ-glutamyl transpeptidase activity (γGTP), urinary protein excretion(UprotV) were assayed. Results eSS rats had elevated HbA1c, Tri, Chol and reduced body weightvs. Wistar control group. Renal function was normal all along the experimental period. TheeSS+AA group also showed elevation of HbA1c, Trig, with no change in Cre and Chol. DuringNNaD SBP was 119±3mmHg and after HNaD 125±1mmHg (p