INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
The effect of freeze-drying on the nutrient, polyphenol and oxidant levels of breast milk
SORIA EA; CORTEZ MV
Mary Ann Liebert, Inc. publishers
Año: 2016 vol. 11 p. 551 - 551
Objectives: Human milk banks need to extend the suitability of milk for breastfeeding, and for this technological advances are required. Our aim was to establish the capacity of freeze-drying to conserve milk properties without further oxidative deterioration. Methods: One hundred sixteen healthy women participated from the city of Cordoba (Argentina). Proteins, glucose, triglycerides, polyphenols, and markers (nitrites, superoxide anion, hydroperoxides, lipoperoxides, and c-glutamyl transpeptidase) were measured in their fresh milk. Samples were then separated for three treatments as follows: freezing and conservation for 6 months at -80C (F: positive control); freeze-drying for 24 hours at £-70C and £1.33 Pa and conservation for 6 months at 4C (FD: treatment of interest); and freeze-drying for 24 hours at £-70C and £1.33 Pa and conservation for 6 months at -80C (FD+F). Next, analyses were repeatedand compared by ANOVA and Tukey tests. Results: Fresh milk showed these values per L as follows: proteins: 12.62? 2.51 g, glucose: 4.44 ? 0.25 g, triglycerides: 34.26 ? 0.59 g, polyphenols: 53.27 ? 8.67 mg, nitrites: 62.40? 19.09 mg, superoxide: 3,721.02 ? 198.80 OD, hydroperoxides: 7,343.76 ? 294.53 OD, lipoperoxides: 7,349.72? 398.72 OD, and c-glutamyl transpeptidase: 4.66 ? 0.55 IU. Glucose was decreased after F treatment ( p < 0.05), all variables were conserved by FD and were not improved by the FD + F combination. Conclusions: Freeze-drying achieved suitable conservation and may improve bank functioning, by protecting nutritional properties, polyphenol-related functionality, and oxidative integrity of human milk through a 1-day treatment with easy maintenance.