INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
Daily Variations in the Glycerol-3-Phosphate Dehydrogenase Isoforms Expression in Triatoma infestans Flight Muscles
MARIA M. STROPPA, CARLOTA S. CARRIAZO, NELIA M. GEREZ DE BURGOS, AND BEATRIZ A. GARCIA
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
AMER SOC TROP MED & HYGIENE
Lugar: Stanford; Año: 2014 vol. 91 p. 399 - 399
Triatoma infestans, the main vector of Chagas disease, is a blood-sucking insect. Flight dispersal of adults is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3- phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. In this study, we explored the profile of GPDH expression in females and males adult flight muscles under light/dark cycle, constant light, and constant dark conditions. Under constant dark conditions, GPDH-1 flight muscles of T. infestans showed a rhythmic pattern of transcription synchronous with a rhythmic profile of activity suggesting regulation by the endogenous circadian clock. Otherwise, the GPDH-2 expression analysis showed no regulation by the endogenous clock, but showed that an external factor, such as the dark/light period, was necessary for synchronization of GPDH-2 transcription and activity.