INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
Differential expression of glycerol-3-phosphate dehydrogenase isoforms in flight muscles of the Chagas disease vector Triatoma infestans (Hemiptera, Reduviidae).
MARÍA M. STROPPA; MARIANA S. LAGUNAS; CARLOTA S. CARRIAZO; BEATRIZ A. GARCÍA; GREGORIO IRAOLA; YANINA PANZERA; NELIA M. GEREZ DE BURGOS
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
AMER SOC TROP MED & HYGIENE
Lugar: Stanford; Año: 2013 vol. 88 p. 1146 - 1146
Flight muscles of Triatoma infestans present two glycerol-3-phosphate dehydrogenase (GPDH) isoforms; GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. The GPDH total activity was greater in the natural population and was almost only due to GPDH-1. The different expression and activity observed between GPDH isoforms in natural population and first laboratory generations wasn`t detected in the second laboratory generation. This pattern may be originated by gradual adaptation to laboratory nutritional conditions. During the development, the expression of GPDH-2 isoform always increases with a longer time of intake, which would imply an increment in the lipid biosíntesis. The GPDH-1 transcript predominated with respect to GPDH-2 in the lower nutritional condition, suggesting the necessity of insects to fly in this nutritional status. The transcriptional pattern revealed a delay at 22°C. The isoforms activities and transcript patterns in flight muscles suggest transcriptional adaptation to metabolic requirements originated by alternative splicing.