Phenotypic and phylogenetic characterization of native peanut Bradyrhizobium isolates obtained from Córdoba, Argentina

VANINA MUÑOZ; FERNANDO IBAÑEZ; MARIA LAURA TONELLI; LUCIO VALETTI; MARIA SOLEDAD ANZUAY; ADRIANA FABRA

SYSTEMATIC AND APPLIED MICROBIOLOGY

ELSEVIER GMBH

Año: 2011

0723-2020

Peanut is an economically important legume nodulated by slow-growing bacteria of the genus  Bradyrhizobium. In this study, a collection of native slow-growing peanut rhizobial isolates from Argentina was obtained and characterized. The phenotypical characterization included the determination of the symbiotic properties; while the genetic and phylogenetic diversity was assessed through ERIC-PCR and sequencing of the internal transcribed spacer (ITS) region and dnaK and nodA genes. The results obtained indicated that peanut nodulating bradyrhizobia are  phenotypically and genotypically diverse, and include locally adapted variants of B. yuanmingense and B. iriomotense carrying novel nodA alleles. Bradyrhizobium. In this study, a collection of native slow-growing peanut rhizobial isolates from Argentina was obtained and characterized. The phenotypical characterization included the determination of the symbiotic properties; while the genetic and phylogenetic diversity was assessed through ERIC-PCR and sequencing of the internal transcribed spacer (ITS) region and dnaK and nodA genes. The results obtained indicated that peanut nodulating bradyrhizobia are  phenotypically and genotypically diverse, and include locally adapted variants of B. yuanmingense and B. iriomotense carrying novel nodA alleles.