INVESTIGADORES
IBAÑEZ Fernando Julio
artículos
Título:
A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants
Autor/es:
J. ANGELINI; F. IBAÑEZ; T. TAURIAN; M.L. TONELLI; L. VALETTI; A. FABRA
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2011 vol. 62 p. 1752 - 1759
ISSN:
0343-8651
Resumen:
In this study, bacteria hosted in root nodules of
single plants of legume Arachis hypogaea L. (peanut) cv
Tegua Runner growing at field were isolated. The collection
of nodule isolates included both fast and slow growing
strains. Their genetic diversity was assessed in order to
identify the more frequently rhizobial strain associated to
nodules from single plants. Molecular fingerprinting of 213
nodular isolates indicated heterogeneity, absence of a
dominant genotype and, therefore, of a unique strains
highly competitive. Efficient nitrogen-fixing isolates were
identified as Bradyrhizobium sp. by phylogenetic analysis
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
Tegua Runner growing at field were isolated. The collection
of nodule isolates included both fast and slow growing
strains. Their genetic diversity was assessed in order to
identify the more frequently rhizobial strain associated to
nodules from single plants. Molecular fingerprinting of 213
nodular isolates indicated heterogeneity, absence of a
dominant genotype and, therefore, of a unique strains
highly competitive. Efficient nitrogen-fixing isolates were
identified as Bradyrhizobium sp. by phylogenetic analysis
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
Tegua Runner growing at field were isolated. The collection
of nodule isolates included both fast and slow growing
strains. Their genetic diversity was assessed in order to
identify the more frequently rhizobial strain associated to
nodules from single plants. Molecular fingerprinting of 213
nodular isolates indicated heterogeneity, absence of a
dominant genotype and, therefore, of a unique strains
highly competitive. Efficient nitrogen-fixing isolates were
identified as Bradyrhizobium sp. by phylogenetic analysis
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
Arachis hypogaea L. (peanut) cv
Tegua Runner growing at field were isolated. The collection
of nodule isolates included both fast and slow growing
strains. Their genetic diversity was assessed in order to
identify the more frequently rhizobial strain associated to
nodules from single plants. Molecular fingerprinting of 213
nodular isolates indicated heterogeneity, absence of a
dominant genotype and, therefore, of a unique strains
highly competitive. Efficient nitrogen-fixing isolates were
identified as Bradyrhizobium sp. by phylogenetic analysis
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.
Bradyrhizobium sp. by phylogenetic analysis
of the sequences of their 16S rRNA genes. The genetic
diversity of 68 peanut nodulating isolates from all the
collected plants was also analyzed. Considering their
ERIC-PCR profiles, they were grouped in eighteen different
OTUs for 60% similarity cut-off. Results obtained in
this study indicate that the genetic diversity of rhizobia
occupying nodules from single plant is very high, without
the presence of a dominant strain. Therefore, the identification
of useful peanut rhizobia for agricultural purposes
requires strongly the selection, among the diverse population,
of a very competitive genotype in combination with a
high-symbiotic performance.