INVESTIGADORES
IBAÑEZ Fernando Julio
artículos
Título:
SELECTION AND IN VITRO CHARACTERIZATION OF BIOCONTROL AGENTS
Autor/es:
M.L. TONELLI; T. TAURIAN; F. IBÁÑEZ; J. ANGELINI ; A. FABRA
Revista:
JOURNAL OF PLANT PATHOLOGY
Editorial:
EDIZIONI ETS
Referencias:
Año: 2010 vol. 92 p. 73 - 82
ISSN:
1125-4653
Resumen:
Over 193 endophytic and epiphytic bacteria were
isolated from different organs of peanut plants growing
under field conditions. The diversity of the isolates was
analyzed by ERIC/BOX-PCR and 16S ribosomal DNA
sequencing. Their potential to protect peanut plants
against the fungal pathogens Sclerotinia sclerotiorum, S.
minor, Sclerotium rolfsii and Fusarium solani was evaluated
by assaying important biocontrol traits. Of the isolates,
148 produced siderophores, 71 (most of them epiphytes)
showed fungal growth inhibition in YEMA
plates, while only 28 also exhibited this activity in PDA
medium. The cell free-extract from 11 bacterial cultures
showed antibiotic activity suggesting the secretion of
soluble extracellular compounds, some being heat-labile
compounds with mol. wt greater than 12 kDa. Fungal
sensitivity to volatile compounds produced by isolates
differed from those observed for soluble secreted compounds
or bacterial cells.Sclerotinia sclerotiorum, S.
minor, Sclerotium rolfsii and Fusarium solani was evaluated
by assaying important biocontrol traits. Of the isolates,
148 produced siderophores, 71 (most of them epiphytes)
showed fungal growth inhibition in YEMA
plates, while only 28 also exhibited this activity in PDA
medium. The cell free-extract from 11 bacterial cultures
showed antibiotic activity suggesting the secretion of
soluble extracellular compounds, some being heat-labile
compounds with mol. wt greater than 12 kDa. Fungal
sensitivity to volatile compounds produced by isolates
differed from those observed for soluble secreted compounds
or bacterial cells., Sclerotium rolfsii and Fusarium solani was evaluated
by assaying important biocontrol traits. Of the isolates,
148 produced siderophores, 71 (most of them epiphytes)
showed fungal growth inhibition in YEMA
plates, while only 28 also exhibited this activity in PDA
medium. The cell free-extract from 11 bacterial cultures
showed antibiotic activity suggesting the secretion of
soluble extracellular compounds, some being heat-labile
compounds with mol. wt greater than 12 kDa. Fungal
sensitivity to volatile compounds produced by isolates
differed from those observed for soluble secreted compounds
or bacterial cells.