Flow cytometry to evaluate Anaplasma marginale parasitemia using a fluorescent nucleic acid stain

MORETTA R; RUYBAL P; MESPLET M; PETRIGH R; NUÑEZ PABLO; GIL G; WILKOWSKY S; GARBOSSA G; FARBER M

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.

Wiley Interscience

Lugar: New york; Año: 2008 vol. 1149 p. 111 - 113

0077-8923

In this work we describe a flow cytometry based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale?infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.