EFFECT OF THE MEDIUM TALP ON THE SELECTION OF PIG SPERMATOZOAS THROUGH THE USE OF HYDROGELS

MORILLA G; A C. LIAUDAT; BLOIS D; CAPELLA V; RIVAROLA C; BARBERO C; BOSCH P; RODRIGUEZ N

Mar del Plata

Congreso; Reunión Anual SAIC SAFE SAB SAP 2019; 2019

EFFECT OF THE MEDIUM TALP ON THE SELECTION OF PIG SPERMATOZOAS THROUGH THE USE OF HYDROGELSMorilla G (1)*, Liaudat AC (1), Blois D (1), Capella V (1,2), Rivarola C (2), Barbero C (2), Bosch P (1) Rodríguez N (1). *gmorilla@ayv.unrc.edu.ar. Dpto de Biol Mol y (2) Dpto de Qca, Fac de Cs. Exactas Fco-Qcas y Naturales, UNRC. In order to select sperm cells with high fertilizer quality, PNIPAM copolymerized with APTA-15% hydrogel surfaces were used. This biomaterial is becoming very important due to its low cellular toxicity, positive vet charge and its great versatility. The aim of this work was to evaluate the binding and release capacity of pig sperm to surfaces of PNIPAM co-APTA-15% in TALP-Ca ++ and TALP- without Ca ++. Initially, the effect of sperm manipulation on sperm viability of was evaluated by exposure to the TALP-Ca ++ medium, without the presence of the hydrogel. In order to evaluate the effect of calcium on cell adhesion, pig sperm were exposed to PNIPAM co-APTA-15% hydrogels surfaces, in TALP-Ca ++ and TALP- medium without Ca ++ for 30. Subsequently the medium was replaced by TALP-Ca ++ and TALP-without Ca ++ in order to analyze whether the cation affects sperm cells relase from hydrogel. Results were statistically analysed by one way ANOVA and Bonferroni as a post-hoc test (p≤0.05). The results suggested that the presence of Ca ++ in the manipulation medium increased sperm cells adhesion to hydrogel surfaces and the absence of this cation released the cells of the PNIPAM co-APTA 15% surfaces. In addition it was found that the hydrogel did not cause any alteration on the sperm viability, compared to the initial semen sample. These results suggest that the presence of calium in sperm manipulation medium affect pig spermatozoa binding and release to PNIPAM co-APTA 15% hydrogel surfaces.