Effect of early stimulations on hypothalamic-Pituitary axis and some immune parameters in prenatal stress rats.

LIAUDAT, A C; RODRIGUEZ N; MAYER N; GAUNA H

Huerta Grande Cordoba

Congreso; Primera Reunión Conjunta de las Sociedades de Biología de la República Argentina; 2007

Sociedad Argentina de Biología

EFFECT OF EARLY STIMULATIONS ON HYPOTHALAMIC-PITUITARY-ADRENAL AXIS AND SOME IMMUNE PARAMETERS IN PRENATAL STRESS RATS. Liaudat A, Rodriguez N, Mayer N, Gauna H F. Fisiología Animal. UNRC. Río Cuarto. Córdoba. nmayer@exa.unrc.edu.ar Prenatal stress (PS) produces an alteration of hypothalamic-pituitary-adrenal (HPA) axis that would induce a long-term alteration of immune function in the offspring. Early postnatal stimulations (M) produce beneficial effect on the long-term emotional reactivity and HPA axis activity that could revert the effect of PS. The objective of this work was to investigate the effect of M in PS animals on HPA axis, the distribution of the subpopulations of leucocytes and the proliferation of lymphocytes T in vitro in response to acute stress in rats. Males of three months of age were used, PS for immobilization (IMO) and controls (PC) during pregnancy. Half of the PS animals were M during the first week of life. Animals of both groups were IMO stressed (20 minutes). Was extracted blood at 0, 20, 60, 90, 120, 150 and 330 minutes, to count white blood cells, the subpopulations of leucocytes and the levels of corticosterone (COR). Thus the spleen of animals was removed for lymphocytes T culture. Profile COR, leucocytes, lymphocytes and neutrophils is similar en PS and PC, however the response is depressed by white blood cells and increased by COR and T- cells proliferation in M animals. In conclusion, M reverts the effects of PS with re-exposure to the same postnatal stressor on: a) The activity of HHA axis, b) The dynamic of leucocytes distribution, which could improve the immune surveillance for pathogens, c) The functional response of T lymphocytes.