Analysis of hydrogel surfaces hyaluronic acid semi-interpenetrating for bovine sperm selection

BLOIS D; A C. LIAUDAT; MORILLA G; BONINO R; CAPELLA V; ALESSIO A; RIVERO R; RIVAROLA C; BARBERO C; RODRIGUEZ N; BOSCH P

Congreso; XXI JORNADAS CIENTÍFICAS Sociedad de Biología de Córdoba; 2017

ANALYSIS OF HYDROGEL SURFACES ASSOCIATED WITH HYALURONIC ACID FOR BOVINE SPERM SELECTION Blois D(1), Liaudat AC(1)*, Morilla G(1), Bonino R(1), Capella V (1,2), Alessio A (1), Rivero R(2), Rivarola C (2), Barbero C (2), Rodríguez N (1), Bosch P (1)(1)Dpto de Biol Mol y (2) Dpto de Qca, Fac de Cs. Exactas Fco-Qcas y Naturales, UNRC. *aliaudat@exa.unrc.edu.com.arMost strategies to separate bovine sperm from seminal fluid and select subpopulations with high fertilization capacity are based on sperm motility and vitality. In addition, there are emerging methods of separation that are based on sperm membrane integrity and cellular ultra-morphology. Hyaluronic acid (HA) sperm selection technique focus on HA receptors found in mature spermatozoa with normal morphological and motile characteristics. The aim of this work was to develop new polymeric biomaterials with HA units that work as support and binding substrate for bovine spermatozoa with high fertilization capacity. Hydrogels polymers were synthesized with HA and the degree of sperm / surface binding was analyzed by phase contrast microscopy. Motility, morphology and viability aspects of attached sperm population were studied. The results indicate that 30% of the spermatozoa exposed to the hydrogels attached to the surfaces and after hyaluronidase treatment, 50% of them were released to the medium. Released sperm cells had good linear movement and viability. These results indicate that polymeric surfaces associated with HA could be used to select high quality spermatozoa for use in assisted fertilization techniques.