Effect of early stimulations on some immune parameters in prenatal stress rats.

LIAUDAT, A C; SARANDÓN A; RODRIGUEZ N; GRECO C; VIVAS A; GAUNA H; MAYER N

Ouro Preto Brasil.

Congreso; 8º Simposio Internacional de Inmunología Veterinaria [8º IVIS],; 2007

Effect of early stimulations on some immune parameters in prenatal stress rats. Ana Liaudat, Aurelia Sarandón ,Nancy Rodriguez, Cecilia R. Greco , Adriana B. Vivasa Hector F.Gauna and Nora Mayer aFacultad de Agronomía y Veterinaria. Facultad de Ciencias Exactas. Universidad Nacional de Río Cuarto. 5800 Río Cuarto. Argentina. cgreco@exa.unrc.edu.ar The application of stressors during pregnancy produces an alteration of the hypothalamic-pituitary-adrenal (HPA) axis that would induce a long-term alteration of the immune function in the offspring. Early postnatal stimulations produce beneficial effect on the long term emotional response and HPA axis activity that could revert the effect of prenatal stress. The aim of this study was to investigate the effect of early stimulations in prenatal stressed animals through the distribution of the subpopulations of leucocytes and the in vitro proliferations of lymphocytes T in response to acute stress in rats. For the study we used: three months old male offsprings from immobilization (IMO) streesed mothers(EP) and offprings from non stressed mothers.(CP). Half of the EP animals were manipulated (M) during the first week of life. Before extraction of blood for basal determinations, the animals of both groups were under acute stress IMO (20 minutes). Then, blood was extracted at 20, 60, 90, 120, 150 and 330 minutes post-stress to count white blood cells, the subpopulations of leucocytes and the levels of corticosterone. Thus, the spleens of all animals were removed for lymphocytes T culture. The profile of the leucocytes, lymphocytes and neutrophils was similar in EP and C, however response was depressed in M animals under postnatal IMO. The in vitro proliferations of lymphocytes T increased in development of M animals. In conclusion, postnatal stimulation reverts the effects of prenatal stress on the distribution of the subpopulations of leucocytes and on the in vitro development of lymphocytes T under the same postnatal stress.