Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems

FERNANDEZ L; REVIGLIO AL; HEREDIA D; MORALES G; SANTO, MARISA; OTERO, LUIS; ALUSTIZA, FABRISIO; LIAUDAT AC; BOSCH, PABLO; LARGHI E; BRACCA A; KAUFMAN T

Heliyon

Elsevier

Año: 2021

2405-8440

Langmuir and Langmuir-Blodgett films holding a synthetic bioinspired wound healing activecompound were used as drug-delivery platforms. Palmitic acid Langmuir monolayers wereable to incorporate 2-methyltriclisine, a synthetic Triclisine derivative that showed woundhealing activity. The layers proved to be stable and the nanocomposites were transferred tosolid substrates. Normal human lung cells (Medical Research Council cell strain 5, MRC-5)were grown over the monomolecular Langmuir-Blodgett films that acted as a drug reservoirand delivery system. The proliferation and migration of the cells were clearly affected by thepresence of 2-methyltriclisine in the amphiphilic layers. The methodology is proposed as asimple and reliable model for the study of the effects of bioactive compounds over cellularcultures.