Potential use of plant-derived volatiles to enhance Anastrepha fraterculus male sexual competitiveness for SIT

RUIZ, M. JOSEFINA; JUÁREZ, M. LAURA; FLAVIA JOFRÉ-BARUD; GOANE, L.; GABRIELA VALLADARES; BACHMANN, GUILLERMO E.; BELLIARD, SILVINA A.; PÁEZ-JEREZ, PAULA; ANA LUCÍA ZAMORA BELLI; MÉNDEZ, DANIELA; JIMÉNEZ, YESSICA; JIMÉNEZ,YOHANA; AMAYA, SOLEDAD; SEGURA DIEGO F.; LOPEZ, LIZA; VERA, MARÍA T

Tapachula

Simposio; 10th International Symposium on Fruit lies of Economic Importance.; 2018

Background: Use of plant-derived volatiles exposure to enhance male mating success has been explored in several fruit fly species. This study evaluated mating competitiveness in Anastrepha fraterculus males exposed to plant-derived volatiles. First we studied the effect of seven essential oils and two synthetic compounds under laboratory conditions and then confirmed the results obtained in field cages simulating an SIT context. Methods: In the laboratory we tested essential oils from ginger (Zingiber officinale), lemon (Citrus limon), orange (C. sinensis), grapefruit (C. paradisi), Schinus polygama, S. areira and Baccharis spartioides and limonene and citral. Mating tests were carried out in 1-L mating arenas, where one exposed laboratory male and one non-exposed laboratory male were caged with one laboratory virgin female. Males were fed brewer?s yeast and sugar (1:3 ratio). Mating pairs obtained by each type of male were recorded for each exposure treatment. In the field cage test we evaluated one essential oil and one compound that enhanced male mating success under laboratory conditions. Laboratory males were fed either hydrolysed yeast and sugar (1:12 ratio) or brewer?s yeast and sugar (1:3 ratio). Thirty exposed laboratory males and 30 non-exposed wild males obtained from infested guavas were caged with 30 wild virgin females in a field cage with two small citrus trees inside. Wild males were fed hydrolyzed yeast. Mating couples were scored early in the morning.Results: Laboratory tests showed that males exposed to the volatiles of C. limon and S. polygama essential oils as well as to limonene achieved significantly more matings than non-exposed males. Males exposed to citral achieved less matings than non-exposed males. Males exposed to the other essential oils volatiles achieved a similar amount of matings than non-exposed males. Under field cage conditions, volatile exposure did not result in an increase of male mating competitiveness. Males fed with hydrolysed yeast exhibited better performance than males fed brewer?s yeast regardless whether they were exposed or not. Conclusions: The differences between the results of the laboratory and the field cage tests could be attributed to differences in the experimental conditions. Therefore, the potential use of plant-derived volatiles exposure to enhance male mating competitiveness in A. fraterculus males needs further investigation. The effect of the adult diet supports including an adequate protein source before male release. Together, we provide information relevant for the development of the SIT against A. fraterculus.