Identification of volatile compounds obtained from sources attractive to the fruit fly parasitoid Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae)

DEVESCOVI, ; FERNANDEZ, ; BACHMANN, ; NUSSENBAUM, ; SCHIAVO; SEGURA

Congreso; 5th International Entomophagous Insects Conference; 2017

Females of Females of Females of Females of Females of Females of Diachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudata Diachasmimorpha longicaudataDiachasmimorpha longicaudata Diachasmimorpha longicaudata Diachasmimorpha longicaudataDiachasmimorpha longicaudata Diachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudata Diachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudataDiachasmimorpha longicaudata Diachasmimorpha longicaudataDiachasmimorpha longicaudata are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host are able to locate their host habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies habitat using chemical information. Previous studies using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary using a stationary olfactometer olfactometer olfactometer olfactometer (no air flux no air fluxno air fluxno air fluxno air fluxno air flux no air flux ) have shown that have shown thathave shown thathave shown thathave shown that have shown that have shown that have shown thathave shown that an orange infested orange infested orange infested orange infested orange infested orange infested orange infested orange infested withwithwith concealed concealed concealed concealed concealed concealed Ceratitis capitata Ceratitis capitataCeratitis capitata Ceratitis capitata Ceratitis capitata Ceratitis capitataCeratitis capitataCeratitis capitataCeratitis capitataCeratitis capitataCeratitis capitataCeratitis capitata (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, (Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae,(Diptera: Tephritidae) larvae, an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but an infested orange but esc aped larvaeaped larvaeaped larvae aped larvaeaped larvaeaped larvaeaped larvaeaped larvaeaped larvaeaped larvae, and a and a and a and a and a rotten (nonrotten (non rotten (non rotten (non -infested) orange infested) orange infested) orange infested) orange infested) orange infested) orange infested) orange infested) orange were preferred by were preferred bywere preferred bywere preferred by were preferred bywere preferred bywere preferred bywere preferred bywere preferred bywere preferred bywere preferred by female female female parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered parasitoids when offered in a in a in a in a dual dual dual dual choice test choice test choice test choice test choice test choice test with a healthy orange with a healthy orange with a healthy orangewith a healthy orangewith a healthy orange with a healthy orangewith a healthy orange with a healthy orangewith a healthy orange with a healthy orangewith a healthy orangewith a healthy orangewith a healthy orange (control) (control) (control) (control). In the present study In the present study In the present studyIn the present studyIn the present study In the present study In the present study In the present studyIn the present study, we , we confirmconfirm confirmconfirmconfirmed the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation the chemical basis of orientation behavior of behavior of behavior of behavior of behavior of behavior of behavior of D. longicaudata D. longicaudataD. longicaudataD. longicaudata D. longicaudataD. longicaudataD. longicaudataD. longicaudataD. longicaudata D. longicaudataD. longicaudata to wards wards wards wards wards these these type of type of type of type of fruit fruit fruit by using a dynamic Y using a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Yusing a dynamic Y using a dynamic Y using a dynamic Yusing a dynamic Yusing a dynamic Y-tube olfactometer tube olfactometer tube olfactometer tube olfactometer tube olfactometer tube olfactometer , suggesting that air suggesting that airsuggesting that airsuggesting that air suggesting that air suggesting that air suggesting that air suggesting that airsuggesting that airsuggesting that air-borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for borne compounds are responsible for the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic the attractiveness. We then collected volatile compounds by dynamic head head-space sampling using HayeSep space sampling using HayeSepspace sampling using HayeSepspace sampling using HayeSep space sampling using HayeSep space sampling using HayeSep space sampling using HayeSep space sampling using HayeSepspace sampling using HayeSep space sampling using HayeSepspace sampling using HayeSepspace sampling using HayeSepspace sampling using HayeSep -Q traps. Q traps. Q traps. Q traps. Q traps. Compounds eluted Compounds eluted Compounds eluted Compounds eluted Compounds eluted Compounds eluted Compounds eluted Compounds eluted with with with methylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GCethylene chloride were analyzed by GC ethylene chloride were analyzed by GCethylene chloride were analyzed by GCethylene chloride were analyzed by GCethylene chloride were analyzed by GCethylene chloride were analyzed by GC -MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST MS and identified using the NIST librarylibrarylibrarylibrarylibrarylibrarylibrary and Kovatsand Kovats and Kovatsand Kovats and Kovatsand Kovats indexindexindexindex . Quali ualiuali/quanti/quanti/quanti /quanti /quantitative tative tative tative tative chemical chemical chemical chemical chemical chemical differences were found differences were found differences were found differences were found differences were found differences were found differences were found differences were found differences were found differences were found differences were found among these oranges, with three terpeneamong these oranges, with three terpene among these oranges, with three terpeneamong these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpeneamong these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpene among these oranges, with three terpeneamong these oranges, with three terpene s shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: shared by the attractive ones: γ-terpinene terpineneterpineneterpinene , α-terpineol terpineolterpineolterpineol , and , and , and , and one of its isomer, one of its isomer, one of its isomer,one of its isomer, one of its isomer,one of its isomer, one of its isomer, terpinen terpinenterpinenterpinen -4-ol . These . These . These . These findings will findings will findings will findings will findings will findings will findings will findings will findings will be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays be followed by bioassays in search of an effectivein search of an effective in search of an effective in search of an effectivein search of an effective in search of an effective in search of an effectivein search of an effective in search of an effective in search of an effective in search of an effective in search of an effectivein search of an effective bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring bait for monitoring parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs. parasitoid populations in biological control programs.