DYNAMICS OF BIOLOGICAL PARAMETERS DURING THE ADAPTATION OF WILD POPULATIONS OF A. FRATERCULUS (DIPTERA: TEPHRITIDAE) TO LABORATORY CONDITIONS

A PARREÑO; M JURI; C CONTE; LIENDO, M. CLARA; MILLA, FABIÁN; DF SEGURA; , MT VERA; CLADERA, J; LANZAVECCHIA, S.B

Congreso; 8th Meeting of the Working Group on Fruit Flies from the Western Hemisphere; 2012

Anastrepha fraterculus Wied. (Diptera: Tephritidae) is a fruit fly pest native from to? South America. Modern insect pests control methods of insect pests such as the Ssterile Iinsect Ttechnique and Bbiological Ccontrol are based on the release of mass reared insects. Therefore, studies on genetic and biological processes that take place involved during the during adaptation to laboratory conditions and subsequently mass-rearing procedures are required in order to carry out these programs successfully. In the present study, individuals collected from a wild population (named TW) were used to establish five introduced into the laboratory to generate five colonies which were reared under laboratory conditions for 6 generations. At the same time, five colonies where initiated with an already established laboratory strain (named CL) with more than 100 generations under cultureartificial rearing. All the colonies were reared and maintained under the same conditions. Every generation we recorded the following parameters were registered: oviposition period, fertility (egg hatch), percent of adults recovered from pupae, sex ratio and adult mortality. Additionally, at G0, G3 and G6, we evaluated egg viability cual es la diferencia con egg hatch?, pupae pupal weight and we performed a male? mating performance analysis. Our results suggest that TW and CL followed an intrinsic dynamic given that considerable significant differences were obtained found between strains TW and CL in every parameter measured, with the except fortion of Ssex Rratio. Both origins strains showed an initial decline decrease for all the measured parameters from G0 to G3 which was then followed by a general increase at G4, and a final stabilization from G5 onwardsto G6 (bueno, si medimos hasta la 6ta entonces deberiamos ser mas suaves). This suggests that after a critical initial bottleneck effect due to the adaptation to new environmental conditions, selected specimens can increase the population quality parameters after three generations G3, reaching the equilibrium in values in G5.mmmm no deberiamos tener mas que g6 p decir esto? Records from tThe Mmating Pperformance test, showed that at G0 females from both lines (TW and CL) preferred the adapted CL males for mating. This is unexpected and an interpretation of these results wcould show that laboratory males are actually more competitive than wild males, or, more likely be that TW males were stressed under the experimental conditions (laboratory conditions). In tAhe same test performed at G5, there were was no signal of significant differences in the election of CL females preference between CL and TW males, but TW females preferred TW males. This suggests that females from recently adapted strains still can still recognize discriminate between males coming from a recently and historically adapted strains and a strains that has been reared for >100 generations, while CL cannot -ojo, hablar con diego-. EvidentlyApparently, this ability is lost in laboratory females where most other parameters remained constant. The results presented here contribute to a better understanding of the adaptation processes of A. fraterculus to laboratory artificial rearing conditions. Additional studies using microsatellite markers are being in progress and will aid in in order to understanding the genetic processes underlying strain adaptation to laboratory conditions.