INVESTIGADORES
SEGURA Diego Fernando
artículos
Título:
Microsatellite markers from the ?South American fruit fly? Anastrepha fraterculus: a valuable tool for population genetic analysis and SIT applications
Autor/es:
SILVIA B LANZAVECCHIA; MARIANELA JURI; ANGELICA BONOMI; LUDVIK GOMULSKI; ALEJANDRA C SCANNAPIECO; DIEGO F SEGURA; ANNA MALACRIDA; GIULIANO GASPERI; JORGE L CLADERA
Revista:
BMC GENETICS
Editorial:
BIOMED CENTRAL LTD
Referencias:
Lugar: Londres; Año: 2014
ISSN:
1471-2156
Resumen:
Background: Anastrepha fraterculus Wiedemann is a horticultural pest which causes significant economic losses in
the fruit-producing areas of the American continent and limits the access of products to international markets. The
use of environmentally friendly control strategies against this pest is constrained due to the limited knowledge of
its population structure.
Results: We developed microsatellite markers for A. fraterculus from four genomic libraries, which were enriched in
CA, CAA, GA and CAT microsatellite motifs. Fifty microsatellite regions were evaluated and 14 loci were selected for
population genetics studies. Genotypes of 122 individuals sampled from four A. fraterculus populations were
analyzed. The level of polymorphism ranged from three to 13 alleles per locus and the mean expected
heterozygosity ranged from 0.60 to 0.64. Comparison between allelic and genotypic frequencies showed significant
differences among all pairs of populations.
Conclusions: This novel set of microsatellite markers provides valuable information for the description of genetic
variability and population structure of wild populations and laboratory strains of A. fraterculus. This information will
be used to identify and characterize candidate strains suitable to implement effective pest control strategies and
might represent a first step towards having a more comprehensive knowledge about the genetics of this pest.