OXIDATIVE STRESS AND SIGNALING PATHWAYS IN ARABIDOPSIS

TELMA E. SCARPECI; MARÍA I. ZANOR; NÉSTOR CARRILLO; ESTELA M. VALLE

Iguazú, Argentina

Simposio; SAIB; 2004

Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular (SAIB)

Molecular mechanisms involved in the early antioxidant response of plants are for the most part still unknown. We examined the expression profile of 22,000 genes of Arabidopsis seedlings subjected to oxidative challenge. Approximately 600 genes showed significant expression changes within the first hours of treatment with methyl viologen, a redox cycling herbicide. The differentially expressed genes included few known proteins implicated in redox processes such us glutaredoxin, thioredoxin, NADH-dependent monodehydroascorbate reductase and ascorbate peroxidase. However, a much larger number of the identified genes have not been previously associated with oxidative stress. Among the highly induced genes were a number of genes related with receptor kinases, calcium regulation, protein modification and transcription factors. Our study revealed the induction of gene expression of signalling pathways common to processes other than oxidative stress, and points to an interplay among the different stress responses of plants at early events.