Effect of daily exposure to aromatic hydrocarbons on folicular growth en ratas

KARINA ANDREA BARREIRO; MARÍA PAULA DI YORIO; ROMINA DOLORES ARTILLO GUIDA; ALICIA GRACIELA FALETTI

Rosario

Congreso; XLI Reunión Anual de la Sociedad Argentina de Farmacología Experimental; 2009

Sociedad Argentina de Farmacología Experimental

Polycyclic aromatic hydrocarbons produce different toxic responses in reproductive health by activating the arylhydrocarbon receptor (AhR). The aim of this project was to study the action of a repeated exposure of two AhR ligands, beta-naphtoflavone (beta-NF) (an AhR agonist) and alfa-naphtoflavone (alfa-NF) (an AhR antagonist) on follicular growth by using immature rats daily treated with different doses (0.1-50 mg/kg) of either beta-NF or alfa-NF. After a 10 day-treatment, the animals were stimulated with gonadotropins (eCG/hCG) to induce ovulation and then killed 20 h after hCG administration. Serum progesterone levels (P), measured by radioimmunoassay, and the developing stages of follicles, determined by histological evaluation, were studied. At high levels, both alfa-NF and beta-NF increased the number of primordial (alfa-NF: 214%; beta-NF: 344%; p