INVESTIGADORES
GARCIA Mariana Gabriela
congresos y reuniones científicas
Título:
A critical role for Galectin-1 in the maintenance of maternal-fetal tolerance
Autor/es:
BLOIS SM; ILARREGUI JM; GARCÍA MG; ÖRSAL A; RABINOVICH G; ARCK P
Lugar:
Córdoba, Argentina
Reunión:
Congreso; VII Congreso Latinoamericano de Inmunología; 2005
Institución organizadora:
Asociación Latinoamericana de Inmunología (ALAI) y Sociedad Argentina de Inmunología (SAI)
Resumen:
Galectin (gal)1, one of the earliestidentified members of a family of lectins, occurs in a variety of tissues undergoing dramatic changes, e.g. the fetoplacental unit. An intriguing function of Gal1 is its modulation of immune responses. Thus, Gal1 is likely a key modulators of pregnancy maintenance. Uterine Gal1 expression was evaluated in uterine tissue from normal, failing and progesteronerescued murine pregnancies by immunohistochemistry. Additionally, mice with failing pregnancies triggered by stress exposure were treated with recombinant Gal1. Phenotype and maturity of dendritic cells (DC) as well as tolerance mechanisms at the fetomaternal interface were also evaluated in the respective groups by flow cytometry and ELISA. The immunomodulatory effects of DCs on allogeneic mixedlymphocyte responses upon Gal1 treatment were also tested in vitro. Uterine expression of Gal1 is downregulated in failing pregnancies and restored by progesterone. Treatment with Gal1 prevents fetal rejection by increasing the presence of an uterine Gal1bright DC population. Subsequently, Gal1bright DC mediate fetal tolerance via a Th2 skew, prevention of Th1 cells, upregulation of indoleamine 2, 3 dioxygenase and CD4+CD25bright T regulatory cells and synthesis of asymmetric antibodies. Our data provide clear evidence for the modulatory function of Gal1 in pregnancy maintenance and suggest Gal1as a promising therapeutic novel agent to improve reproductive outcome.bright DC population. Subsequently, Gal1bright DC mediate fetal tolerance via a Th2 skew, prevention of Th1 cells, upregulation of indoleamine 2, 3 dioxygenase and CD4+CD25bright T regulatory cells and synthesis of asymmetric antibodies. Our data provide clear evidence for the modulatory function of Gal1 in pregnancy maintenance and suggest Gal1as a promising therapeutic novel agent to improve reproductive outcome.
