Differential gene expression associated to migratory, secretory and morphogenesis processes, and VEFG-mediated signaling in osteosarcoma cell lines that differ in their metastatic ability.

GUTIERREZ LM; AMOROS MA ; BAYO J; MAZZOLINI G; KLEINERMAN ES; GARCIA MG; CORREA DOMINGUEZ A; BOLONTRADE MF

Congreso; Reunión anual conjunta de SAIC, SAI, SAFE, NANOMEDAR Y AACYTAL.; 2016

Despite combination of chemo- and radiotherapy, survivalrate for osteosarcoma (OS) remains 60-70%. OS presents majortherapeutic challenges at lung metastasis stage with a survivalrate of 15-30% at diagnosis time. We used a metastatic OS modelthat includes a subline (LM7) with enhanced lung metastatic ability. Proteomic analysis identified differentially expressed proteins both in the secretory and cellular compartments of the metastaticand parental (SAOS2) cell lines. We identified an increased geneexpression associated to VEGF signaling, morphogenesis andtissue remodeling and migratory ability in the metastatic subline.A functional profile evaluation revealed no significant differencesbetween both cell types secretome´s ability to induce angiogenesis in vitro, suggesting that a differential role for VEGF could beassociated to non-angiogenic related mechanisms. LM7 showedincreased in vitro migratory behavior; moreover, mesenchymalstem cells (MSC) displayed enhanced migration towards the parentalsecretome (1200±98 vs 820±158 cells/field, SAOS2 vs LM7respectively, p