Noradrenaline in coeliac ganglion through an indirect effect favors the luteal survival

BRONZI CD; RODRÍGUEZ D; VALLCANERAS S; SOSA ZY; RASTRILLA AM; CASAIS M

Congreso; XXXI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2013

    NORADRENALINE IN COELIAC GANGLION THROUGH AN INDIRECT EFFECT FAVORS THE  LUTEAL SURVIVAL Bronzi CD, Rodríguez D, Vallcaneras S, Sosa ZY, Rastrilla AM, Casais M. Biol. Reprod (LABIR)- FQByF -UNSL - San Luis -Chacabuco 917  cdbronzi@gmail.com   It is known that the ovary is innervated by superior ovarian nerve (SON) whose fibers originate in the Coeliac Ganglion (CG). The objective of this study was to evaluate if Noradrenaline (NA), added in CG, modulates the ovarian release of GnRH, Progesterone (P4) and Oestradiol (E2); besides, the expression of P4 synthesis (3β-HSD) and degradation (20α-HSD) enzymes, and apoptotic factors such as bax/bcl-2, in Diestrous II stage. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments with and without NA (10-6M) added in CG compartment. Extractions of the ovarian incubation liquid were carried out at 60 and 120 min. P4, E2 and GnRH were measured by RIA and the expression of 3β-HSD, 20α-HSD, bax and bcl-2 by RT-PCR. ANOVA 1 followed by Bonferroni test with a statistical significance of (p<0.05) was used. NA in CG decreased ovarian P4 (p<0.001) coinciding with a decrease of 3β-HSD (p<0.01) and a tendency to increases the expression of 20α-HSD, E2 increased and GnRH diminished (p<0.05) levels respect to the control group. With regard of bax this decreased (p<0.01) and bcl-2 increased (p<0.01) while the relationship bax/bcl-2 wasn´t modified respect to the control group. We conclude that NA favors luteal survival through an indirect effect on the ovary via SON. This effect will be independent of GnRH.