The cholinergic agents modify the luteal regression at the first diestrous in the rat

DELSOUC B, ; , VALLCANERAS S; BENEGAS J,; CARRIZO D,; DELGADO SM,; RASTRILLA AM

Congreso; Sociedad de Biología de Cuyo. XXXV Reunión Científica Anual; 2010

The cholinirgic agents modify the luteal regression at the first diestrous in the rat.  Delsouc MB, Vallcaneras S, Benegas J, Carrizo D, Delgado SM, Rastrilla AM. Lab Biol de la Reproducción. Fac Qca Bqca y Farmacia. Univ Nac de San Luis. 5700. E-mail: sdelgado@unsl.edu.ar   Using the ex vivo coeliac ganglion-superior ovarian nerve-ovary (CG-SON-O) system, we have shown that the CG stimulation with cholinergic agents, modifies the secretion of ovarian steroids in rats at different stages of the reproductive life. The aim was to elucidate the effect of Acetylcholine (Ach), as cholinergic agonist, Atropine and Hexametonium, as muscarinic and nicotinic antagonist respectively in CG on the luteal regression at the first diestrous of estrous cycle. The system was incubated in Krebs Ringer-glucose at 37ºC. The cholinergic agents (all in 10-6M concentration), were added in the CG compartment. Controls were not stimulated. Release of ovarian Progesterone (P) was measured by RIA at 15, 30, 60 and 120 min. At 120 min, ovarian mRNA expression of bax and bcl-2 (apoptosis regulators) were assessed by RT-PCR. ANOVA 1 followed by Tuckey test with a statistical significance of p<0.05 was used.  The addition of Ach and Hexametonium in CG, decreased the release of ovarian P at all the time. Atropine decreased the P at 15´, 30´y 60´and increased it at 120´. The three cholinergic agents tested increased the expression of bax and bcl-2 and decreased the ratio of bcl-2: bax respect to Control. The ganglion cholinergic stimulus in the first diestrus favours the apoptosis induction.