Estradiol in celiac ganglion, modulates the luteal regression at the end of pregnancy in rat

CAMPO VERDE ARBOCCO F; VALLCANERAS S; HAPON B; CASAIS M; RASTRILLA AM

Congreso; Sociedad de Biología de Cuyo XXVII Reunión Científica Anual; 2009

ESTRADIOL IN CELIAC GANGLION,  MODULATES THE LUTEAL REGRESSION AT THE END OF PREGNANCY IN THE RAT Campo Verde Arbocco F, Vallcaneras S,  Hap¨®n B, Casais M, Rastrilla AM Laboratorio de Biolog¨ªa de la Reproducci¨®n-FQByF-UNSL Chacabuco 917   Estradiol (E2) is a key hormone in the regulation of reproductive processes acting both on peripheral organs and sympathetic neurons associated to reproductive functions. We found, using the ex vivo Celiac Ganglion-Superior Ovarian Nerve-Ovary (CG-SON-Ovary) system, that E2 added to the CG  inhibited the liberation of progesterone (P) in ovary but did not modify the activity of 3¦Â-hydroxysteroid dehydrogenase (3¦Â-HSD) and 20¦Á-HSD (synthesis and degradation P enzymes, respectively). The objective of this work was to research, in the same experimental scheme, if E2 in CG modifies the luteal regression. The ex vivo CG-SON-Ovary system was incubated in metabolic bath, keeping CG and ovary connected by the SON, in separate cuvettes. In CG compartment E2 was added in 10-6M, 10-8M and 10-9M concentrations. Controls were not stimulated. After 240 min of incubation, RNA was extracted from luteal tissue to determine the expression of 3¦Â-HSD, 20¦Á-HSD, Bcl-2 (antiapoptotic) and Bax (proapoptotic) genes by RT-PCR. Was utilized  ANOVA 1 followed by Tuckey test with a statistical significance of p <0.05. E2 in CG did not modify neither 3¦Â-HSD, 20¦Á-HSD, Bcl-2, Bax expression in any concentrations tested. But E2  in 10-8M and 10-9M concentrations increased the ratio of Bcl-2/Bax. E2 in CG, through the SON, might prevent the onset of structural luteal regression still when the ovarian P is disminished.