Diferente respuesta del ovario prepuberal y del primer proestro al estímulo colinérgico ganglionar

DELGADO SM; CASAIS M; VALLCANERAS S; SOSA Z; RASTRILLA AM

Huerta Grande -Córdoba

Congreso; Primera Reunión Conjunta de Sociedades de Biología de República Argentina. Huerta Grande.; 2007

  DIFFERENT RESPONSE OF PREPUBERTAL AND FIRST PROESTROUS OVARY TO CHOLINERGIC INFLUENCES Delgado SM, Casais M, Vallcaneras S, Sosa Z, Rastrilla AM. Lab. Biol. de la Reproducción. Fac. Qca, Bqca. y Farmacia. Univ Nac. de San Luis. 5700. San Luis. E-mail: sdelgado@unsl.edu.ar The cholinergic effect on the coeliac ganglion (CG) has been described in the prepubertal ovary of rats; yet, it remains unstudied in the first proestrous of the estral cycle. Purpose: to elucidate the effect of Acetylcholine in the ganglion compartment in the ex-vivo CG-superior ovarian nerve-ovary system in rat on the ovarian release of Progesterone (P), androstenedione (A2) and nitric oxide (NO) in the first proestrous and to compare it with the prepubertal ovary. The system was isolated and incubated in Krebs-Ringer-glucose buffer in a metabolic bath in the presence of Ach (10-6 M) in CG. Aliquots from the ovarian compartment were taken at 15, 30, 60 and 120 min. P and A2 were measured by RIA, and NO, by Griess reaction. Statistical significance by Student´s t test at p<0.05. Results: Ach caused a significant increase in P and A2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage. 2) and nitric oxide (NO) in the first proestrous and to compare it with the prepubertal ovary. The system was isolated and incubated in Krebs-Ringer-glucose buffer in a metabolic bath in the presence of Ach (10-6 M) in CG. Aliquots from the ovarian compartment were taken at 15, 30, 60 and 120 min. P and A2 were measured by RIA, and NO, by Griess reaction. Statistical significance by Student´s t test at p<0.05. Results: Ach caused a significant increase in P and A2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage. -6 M) in CG. Aliquots from the ovarian compartment were taken at 15, 30, 60 and 120 min. P and A2 were measured by RIA, and NO, by Griess reaction. Statistical significance by Student´s t test at p<0.05. Results: Ach caused a significant increase in P and A2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage. 2 were measured by RIA, and NO, by Griess reaction. Statistical significance by Student´s t test at p<0.05. Results: Ach caused a significant increase in P and A2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage. t test at p<0.05. Results: Ach caused a significant increase in P and A2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage. 2 release at all the studied times (p<0.05) in the first proestrous and a decrease at 30 days. NO showed similar values in the first proestrous and at 30 days, and the ganglion cholinergic stimulus increased liberation in both cases (p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic stimulus in the first proestrous favours the ovary maturation through the increase of the hormonal secretion partly due to the low liberation of NO in relation to the prepubertal stage.