INVESTIGADORES
VALLCANERAS Sandra
congresos y reuniones científicas
Título:
Effect of progesterone on luteal regression through a neural pathway in late pregnant rat
Autor/es:
GHERSA F, ; VALLCANERAS S,; RASTRILLA AM,; CASAIS M.
Reunión:
Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012
Resumen:
EFFECT OF PROGESTERONE ON LUTEAL REGRESSION VIA A NEURAL PATHWAY IN LATE
PREGNANT RAT.
Ghersa F, Vallcaneras S, Rastrilla AM, Casais M. Lab. Biol. Reprod (LABIR)-
FQByF -UNSL - San Luis -Chacabuco 917 ssvallca@unsl.edu.ar
Previously, we demonstrated that
Progesterone (P) added to the coeliac ganglion (CG), via the superior ovarian
nerve (SON), is able to stimulate the ovarian Pg release. The object of this
study was to analyze the effect of P, through SON, on the functional and
structural luteal regression of 21 pregnant rats using the ex vivo CG-SON-ovary system.
The system was incubated in Krebs
Ringer at 37ºC,
keeping CG and ovary connected by the SON, in separate compartments. P (10-5M)
was added in the ganglion compartment [(P)g], Controls were not
stimulated. Periodic extractions of the
ovarian incubation liquid were carried out at 30, 60, 120 and 180 min. To corroborate previous results of P, it was determined by RIA. At 180 min, the luteal mRNA expression of
3β-HSD, 20α-HSD (synthesis and degradation enzymes respectively), Fas and Fas-L
were analysed by RT-PCR. ANOVA 1 followed by
Tuckey test with a statistical significance of p<0.05 was used.
[(P)g] increased
ovarian P and decreased the expression of 20α-HSD and FasL
without modifing 3β-HSD and Fas expression. In conclusion, P would promote corpora lutea
protection from regression through the peripheral neural pathway.
Biología del Desarrollo y Reproducción (DR)
San Luis