Effect of progesterone on luteal regression through a neural pathway in late pregnant rat

GHERSA F, ; VALLCANERAS S,; RASTRILLA AM,; CASAIS M.

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

EFFECT OF PROGESTERONE ON LUTEAL  REGRESSION VIA A NEURAL PATHWAY IN LATE PREGNANT RAT. Ghersa F, Vallcaneras S, Rastrilla AM, Casais M. Lab. Biol. Reprod (LABIR)- FQByF -UNSL - San Luis -Chacabuco 917  ssvallca@unsl.edu.ar   Previously, we demonstrated that Progesterone (P) added to the coeliac ganglion (CG), via the superior ovarian nerve (SON), is able to stimulate the ovarian Pg release. The object of this study was to analyze the effect of P, through SON, on the functional and structural luteal regression of 21 pregnant rats using the ex vivo CG-SON-ovary system. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. P (10-5M) was added in the ganglion compartment [(P)g], Controls were not stimulated. Periodic extractions of the ovarian incubation liquid were carried out at 30, 60, 120 and 180 min. To corroborate previous results of P, it was determined by RIA. At 180 min, the luteal mRNA expression of 3β-HSD, 20α-HSD (synthesis and degradation enzymes respectively), Fas and Fas-L were analysed by RT-PCR. ANOVA 1 followed by Tuckey test with a statistical significance of p<0.05 was used. [(P)g] increased ovarian P and decreased the expression of 20α-HSD and FasL without modifing 3β-HSD and Fas expression. In conclusion, P would promote corpora lutea protection from regression through the peripheral neural pathway.   Biología del Desarrollo y Reproducción (DR) San Luis