Ganglionic effect of progesterone on luteal regression in late pregnant rat

GHERSA F ; VALLCANERAS S; DE LA VEGA MAGALI,; RASTRILLA AM; CASAIS M

Mendoza

Congreso; XXXI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2013

GANGLIONIC EFFECT OF PROGESTERONE ON LUTEAL  REGRESSION IN LATE PREGNANT RAT. Ghersa F, Vallcaneras S, De la Vega Magali, Rastrilla AM, Casais M. Lab. Biol. Reprod (LABIR)- FQByF -UNSL - San Luis -Chacabuco 917 ssvallca@unsl.edu.ar   Previously, we demonstrated that Progesterone (P4) added to the coeliac ganglion (CG), via the superior ovarian nerve (SON), stimulated the ovarian P4 release, decreased the luteal expression of 20α-HSD, P4 degradation enzyme and the FasL expression, regulator factor of the way extrinsic of apoptosis. The aim of this study was to analyze the ganglionic effect of P4, through SON, on the  luteal expression of 3β-HSD, P4 synthesis enzyme, Bax  and Bcl-2, regulator factors of the way intrinsic of apoptosis in pregnant  rats  on 21 day using the ex vivo CG-SON-ovary system. The system was incubated in Krebs Ringer at 37ºC, keeping CG and ovary connected by the SON, in separate compartments. P4 (10-5M) was added in the ganglion compartment [(P4)g], At the end of incubation (180 min), the luteal expression of 3β-HSD was studied by Western Blot and the expression of  Bax and Bcl-2  was analysed by RT-PCR. Student´s test with a statistical significance of p<0.05 was used. [(P4)g] did not modify significantly the expression of the parameters of luteal regression studied in this work. In conclusion, the luteotrophic effect of P4 through the peripheral neural pathway would be mediated by inhibition of 20α-HSD enzyme and by modulation of extrinsic pathway of apoptosis.   Biología del Desarrollo y Reproducción (DR) San Luis