Study of the effect of ammonium tetrathiomolybdate on the uterus of mice with induced endometriosis.

ZAPATA F, ; DELSOUC MB,; CONFORTI RA,; ZABALA AS,; VALLCANERAS SS,; CASAIS M.

Mendoza

Congreso; XL Reunión Anual de la Sociedad de Biología de Cuyo; 2022

STUDY OF THE EFFECT OF AMMONIUM TETRATHIOMOLYBDATE ON THE UTERUS OF MICE WITH INDUCED ENDOMETRIOSISZapata F, Delsouc MB, Conforti RA, Zabala AS, Vallcaneras SS, Casais MLaboratorio de Biología de la Reproducción (LaBiR), Universidad Nacional de San Luis (UNSL)/ Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). E-mail: mbdelsouc@unsl.edu.ar Endometriosis (EDT) is a complex estrogen-dependent disease that affects primarily pelvic tissues. It is characterized by the growth of tissue similar to the endometrium outside the uterus, which causes inflammation and the formation of adhesions. Therefore, EDT often causes intense pelvic pain, dysuria, dyspareunia, dysmenorrhea and subfertility, affecting the quality of life of patients. There is no cure for this disease and new treatments are needed to control its progression. It was recently shown that Ammonium Tetrathiomolybdate (TM, copper chelator) inhibits the progression of experimental EDT. However, it is necessary to analyze the possible unwanted effects of the drug on the uterus. The aim of this work was to analyze if the oral administration of TM alters the histology and oxidative state of the uterine tissue of mice with EDT. Eighteen female C57BL/6 mice were divided into three experimental groups: Sham (placebo surgery), EDT, and EDT+TM. EDT induction was performed by autologous transplantation of uterine tissue to the intestinal mesentery. The EDT+TM group received 0.30 mg of TM/day in their drinking water from postoperative day 15. One month after inducing the pathology, intact uterine tissue samples were collected for histological analysis (hematoxylin-eosin stain) and oxidative stress studies (total antioxidant capacity, catalase activity, superoxide dismutase activity, and concentration of malondialdehyde and nitrites). Data were statistically analyzed using one-way ANOVA followed by Tukey´s test (P < 0.05 was considered statistically significant). Interestingly, the analyzed factors did not show significant changes between the experimental groups. It is probable that the observation of possible changes in the uterus requires a longer period of experimentation. However, it is not a minor detail that the administration of TM does not cause an adverse effect on the uterus, despite considerably inhibiting the development of endometriotic-like lesions in the same experimental model. These observations continue to support the study of TM as a possible innovative treatment for EDT. Área temática: Biología del Desarrollo y Reproducción (DR)