INVESTIGADORES
GIL Jose Fernando
artículos
Título:
Susceptibility of different mouse strains to Leishmania amazonensis infection
Autor/es:
FALÚ, MA; GARCÍA BUSTOS, MF; PARODI, C; MOLINA DE RASPI, E; CARDOZO, RM; CIMINO, R; GIL, J; BASOMBRIO, MA
Revista:
Dermatol Argent
Editorial:
Sociedad Argentina de Dermatología
Referencias:
Año: 2009 vol. 15 p. 334 - 339
Resumen:
Background: In the most studied mouse model produced by Leishmania major, association was
found between Th1 and Th2 immune responses, and resistant (C57BL/6) and
susceptible (BALB/c) strains, respectively. This dichotomy is not observed in
models developed with other Leishmania species. Therefore, advancing in the
study of experimental models involving dominant species in our region
represents an important challenge. The purpose was to reproduce the disease in
diff erent mouse strains after infection with L. amazonensis. Methods. To study
the eff ect of the mice strain variable on susceptibility for L. amazonensis
infection, a constant parasite inoculum was applied to the studied strains.
Response to infection was characterized in C57BL/6, BALB/c, and Swiss strains
by measuring lesions, estimating parasite load and histological analysis. Serum
antibodies and cytokines were determinated by ELISA. Statistical analysis:
ANOVA test. Results. BALB/c showed maximum susceptibility to infection, Swiss
demonstrated an intermediate phenotype, and C57BL/6 was the less susceptible strain.
We obtained murine models reproducing diff erent clinical forms comparable to
human disease. Conclusions. The results will be useful to extrapolate the
conclusions of future therapeutic and prophylactic analysis in experimental
models to human pathology