Oxidative stress induced by Atrazine in Palaemonetes argentinus: the protective effect of Vitamin E

JULIETA GRIBOFF; DAVID MORALES; LIDWINA BERTRAND; ROCIO BONANSEA; . MAGDALENA V. MONFERRAN; RAMON ASIS; DANIEL WUNDERLIN; VALERIA AME

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2014 vol. 108 p. 1 - 8

0147-6513

The widespread contamination and persistence of the herbicide atrazine residues in the environment resulted in the exposure of nontarget organisms. The present study was undertaken to investigate the effect of atrazine in the response of oxidative stress biomarkers in the freshwater shrimp Palaemonetes argentinus and the protective effect of vitamin-E against atrazine-induced toxicity. Therefore, two batches of P.argentinus were fed for 21 days with a commercial food enriched in proteins (D1) or with D2, composed of D1 enriched with vitamin-E. Subsequently, half of individuals of each group were exposed to atrazine (0.4 mg L-1) for 24 h and the others remained as controls. Atrazine promoted oxidative stress in P.argentinus fed with D1 as indicated by enhanced H2O2 content and induction of SOD, GSTs and GR. This antioxidant activity would prevent the increment of TBARs in the shrimp tissues. P.argentinus fed with D2 could reverse the response of the biomarkers measured. However, the activation of antioxidants response had an energetic cost, which was revealed by a decrease in lipids and glycogen storage in shrimps. These results show the modulatory effect of vit-E on oxidative stress and its potential use as an effective antioxidant to be applied in chemoprotection strategies during aquaculture.