Immunomodulatory mechanisms exerted by probiotic bacteria in the intestinal mucosa immune system

LEMME DUMIT, JOSÉ MARÍA; SAAVEDRA, LUCILA; HEBERT, MARIA ELVIRA ; PERDIGÓN, GABRIELA; MALDONADO GALDEANO, CAROLINA

Congreso; Reunión Conjunta de Sociedades de BioCiencias; 2017

Probiotics bacteria can interact with mucosal immune cells or epithelial cells lining the mucosa to modulate specific functions of the mucosal immune system. Here, we aimed to study the differences in the mechanisms exerted by probiotic bacteria and its cell wall in the gut mucosa, compared with an enteropathogenic bacteria. Six-wk-old BALB/c mice received in the drinking water a suspension of viable Lactobacillus casei CRL431 (Lc431) (108 CFU/mL) or by gavage its cell wall (CW431) (100 mL/day) for 7 days, d8 animals were sacrificed. One dose of Salmonella Typhimurium (108 CFU/mL) were performed on 6-wk-old BALB/c mice, after 72 h animals were sacrificed. Relative fold change gene expression (A20, IRAK-M, MKP-1, and Tollip) were determined on intestinal epithelial cells (IEC) and on peritoneal macrophages (pMQ) by RT-qPCR. The percentages of Treg and Th17 cells were analyzed on the lamina propria of the gut by flow cytometry. Lc431 and its CW431 were able to increase significantly the regulatory genes expression of NF-KappaB and MAPK pathways on IEC and pMQ compared to Salmonella (p