ORAL ADMINISTRATION OF PROBIOTICS INCREASE ANTIMICROBIAL PEPTIDES PRODUCTION BY PANETH CELLS.

CAZORLA, SILVIA INES; LEMME DUMIT, JOSÉ MARÍA; PERDIGÓN GABRIELA; MALDONADO GALDEANO, CAROLINA

Mar del Plata

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

SAI, SAFE, SAIC, NANOMEDAR,AACYTAL

The huge amount of intestinal bacteria represents a continuing threat to the intestinal barrier. In order to meet this challenge, gut epithelial produce a lot of antimicrobial peptides (AMP) that act at the forefront of innate immunity. The aim of this work was to explore whether AMP production by Paneth cells is influenced by probiotics administration, in order to avoid the imbalance of intestinal microbiota and preserve intestinal permeability.Groups of 45 days old BALB/c mice received Lactobacillus casei CRL 431 (LC 431), Lactobacillus casei CNCM I-1518 (LC 1518) or water upon 7 and 5 days, respectively. At the end of this time mice were sacrificed and samples of intestinal fluids, small and large intestine sections were taken. In mice fed with both probiotics bacterial, an increase in the number of Paneth cells were observed after hematoxylin and eosin stain of small intestine. Besides, an increase in the microbicidal activity against the pathogens S. aureus and S. thyphimurium by these intestinal fluids was observed respect to the group that received conventional diet (p