Probiotic supplementation in re-nutritional diets improves cytokine profile in thymus and intestine of malnourished mice

BALCELLS, FLORENCIA; VELEZ, EVA M. M.; CAZORLA, SILVIA INES; PERDIGÓN, GABRIELA.; FELIÚ, SUSANA; MALDONADO GALDEANO, CAROLINA

San Luis

Congreso; LXXI Reunión Anual de la Sociedad Argentina de Inmunología; 2023

Malnutrition affect the intestinal barrier, specially the intestinal epithelial cells (IEC), and also influence the thymus (T) activity, leading to a dysfunction of the immune response that can significantly increase morbidity and mortality from infections.AIM: to evaluate the effect of nutritional recovery supplemented with different probiotic strain on the microenvironment of T and IEC through the production of cytokines in malnourished mice.Methods: Balb/c mice were divided into groups according to the different diets and supplementation: A) NC: Normal Control; B) DSN: Malnutrition; C) DSN+A: DSN+ conventional food; C) DSN+L: DSN+Milk; D) DSN+Lp: DSN+ Lactobacillus Paracasei 1518; E) DSN+M: DSN+ commercial probiotic MIX; F) DSN+A+L: DSN+ conventional food + Milk; G) DSN+A+Lp: DSN+ conventional food + Lactobacillus Paracasei 1518; and H) DSN+A+M: DSN+ conventional food + commercial probiotic MIX .Samples: T and IEC from mice of all experimental groups were taken and we performed ex vivo cultures: IL-6, IFN-γ, TNF-α, IL-12 and IL-10 levels were measured by ELISA. Statistical analyzes were performed by GraphPad Prism software using the ANOVA, with Tukey's correction for multiple comparisons, p< 0.05 was considered statistically significant.Results: in T and IEC of mice from the DSN group, levels of proinflammatory cytokines increased respect to the normal control, while values of IL-10 decreased. Re-nutrition diets in malnourished mice significantly decreased levels of proinflammatory cytokines and increased IL-10 values in T and IEC respect to DSN mice, these results being more significant in DSN mice receiving Lp and M, even reaching values close to normal control.Conclusion: renutrition diets (with A or L) in DSN mice demonstrated improvement in level of proinflammatory cytokines and IL-10 in T microenvironment and in IEC, however supplementation with Lp and M (alone or combined with re-nutrition diets) showed to be significantly more effective in the recovery of the regulatory action of the T cells and the IEC.These results show that probiotic supplementation could be a valuable tool to help improve the functioning of the immune system in malnourished patients.