INVESTIGADORES
FALCON Cristian Roberto
artículos
Título:
Adoptive transfer of dendritic cells pulsed with Fasciola hepatica antigens and LPS confers protection against fasciolosis in mice
Autor/es:
FALCÓN CRISTIAN; CARRANZA FRANCO; AOKI PILAR; MOTRÁN CRISTINA; CERVI LAURA
Revista:
JOURNAL OF INFECTIOUS DISEASES
Editorial:
UNIV CHICAGO PRESS
Referencias:
Año: 2012 vol. 205 p. 506 - 514
ISSN:
0022-1899
Resumen:
Dendritic cells (DCs) can function as adjuvants able to mediate protection against different pathogens. Giventhat successful vaccination against Fasciola hepatica is mostly related to the induction of Th1 responses, westudied the potential of DCs loaded with F. hepatica antigens and lipopolysaccharide (LPS) (which promoteDCs maturation) as a vaccine against fasciolosis in BALB/c mice. However, only a semimature phenotype wasachieved when DCs were simultaneously cultured with an F. hepatica total extract (TE) and LPS. The activationstatus of TE-loaded DCs was enhanced when these cells were treated with TE 90 minutes before beingstimulated with LPS (TE90 DCs). More importantly, a single vaccination of mice with TE90 DCs stimulateda systemic Th1 response and conferred protection against hepatic damage induced by F. hepatica infection.Thus, TE90 DCs may prove to be a useful new tool for vaccination against F. hepatica.
