In vitro Antibacterial Activity of Dehidroleucodine Against Helicobacter pylori

VEGA, AE., STEGE PW., GH. WENDEL, AOM. MARÍA, L. PELZER, O. GIORDANO.

San Luis

Congreso; XXXV reunion Anual de la Sociedad Argentina de Farmacología Experimental; 2004

SAFE

Dehydroleucodine (DhL), was isolated from Artemisia dougiasiana Besser, known as "matico" and commonly used for its digestive properties. The antibacterial activity of DhL was assayed on six strains of H. pylori isolated from gastric biopsies and the reference strain H. pylori NCTC 11638, and was evaluated by agar dilution method. Serial dilutions of the drugs ranging from 0.008 to 128 mg/l were prepared in Mueller-Hinton agar supplemented with 7% horse blood. Plates were incubated for 3 to 5 days, and the MIC was recorded as the lowest concentration of the drugs inhibiting visible growth. DhL showed good activity against all strains tested with MICs ranging from 1 to 8 mglL. In other study, twenty four hours before the experiment Wistar rats were fasted and the duodenum was ligated (Melchiorri el al., 19(7). Absolute ethanol was employed as ulcerogenic agent. DhL reduced ethanol-induced gastroduodenal damage in rats (p<0.05). The results presented indicate that DhL prevents the formation of gastric and duodenal lesions induced by absolute ethanol in ligated rats, and this compound has significant antimicrobial properties against H. pylori. DhL could represent a usefull tool in relieving digestive disorders.