Prevalence of Antibiotic Resistance of Helicobacter pylori Strains in San Luis

STEGE P; GÓMEZ P; NAVEA D; MAJUL R; VEGA AE

San Luis

Congreso; Biology Society XXI Annual Scientific Meeting, 10 th Aniversary of Biocell.; 2003

Sociedad de biología de cuyo

Helicobacter pylori eradication treatment is indicated in all patients with active o recurrent peptic ulceration. Commonly used antibiot­ics include clarithromycin (CLA), metronidazole (MTZ), amoxycillin (AMX) and telracycline (TET). There are reports of increased antibiotic resistance rates in H. pylori strains around the world; therefore it is important to be informed about the antibiotic resistance of H. pylori locally. The aim of this study was to deter­mine the prevalence of antibiotic resistance of H. pylori strains isolated in San Luis. We studied forty-three strains isolated from symptomatic adult patients. Susceptibility lo MTZ, CLA, AMX and TET was determined by an agar dilution method according to the NCCLS recommendations. H. pylori NCTC 11638 was used as a control. Antibiotics were added to Mueller-Hinton agar supple­mented with 7% horse blood as two-fold dilutions from 128 lo 0.008 mg/L. A strain was considered resistant lo MTZ or CLA when MIC was = 8 mg/L or MIC was = 2 mg/L, respectively. The overall percentage of resistance was 30.2% (13/43) for MTZ and 34.8% (15/43) for CLA. No strain was resistant lo AMX or TET. As the prevalence of antibiotic resistance varies according to the country, local data are necessary in order to choose the best treatment against H. pylori.